Background: Nearly all patients receive intravenous therapy during hospital stay, and as nurses play a piv­otal role in administering of Intravenous fluids, their knowledge of intravenous therapy is critical in pro­moting patient safety and preventing complications for positive patient outcomes.

Objective: We assessed the level of nurses’ knowledge regarding Intravenous fluid therapy at Jackson F. Doe memorial regional referral hospital (JFD).

Methods: An institutional based cross-sectional study was conducted using pretested, semi-structured, self-administered questionnaire among 46 nurses working at Jackson F. Doe hospital. The data was analyzed us­ing SPSS version 20 (independent T-test and ANOVA were used for inferential analysis).

Results: The mean knowledge score on intravenous fluid therapy (IVT) among nurses was 43.48± 15.33%, depicting inadequate knowledge. Satisfactory knowl­edge on intravenous fluid was seen in 22.6% while 67.4% of nurses had inadequate knowledge. Highest knowledge deficiency was observed on ionic compo­sition of fluids, indication of commonly available flu­ids, monitoring parameters of patients’ fluid status, complication of IVT and its preventive measures. Sta­tistically significant association was present between mean knowledge score and gender (p=0.046) and years of experience (P=0.038).

Conclusion: Level of knowledge of intravenous fluid therapy among nurses is inadequate. Continuous ed­ucation and training of nurses on IVT should be con­ducted regularly to improve their knowledge and con­sequently, the quality of patient care. Further research using observational checklist to assess nurses’ practice during intravenous fluid therapy is needed.